ZRB2782

Anti-Gephyrin Antibody, clone 1D12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Domain E, E-domain, EC:2.10.1.1, GPH, KIAA1385, MPT Mo-transferase, Molybdopterin molybdenumtransferase
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D12, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 80 kDa; observed mol wt ~95 kDa
• purified by: using Protein A
• species reactivity: mouse, human, rat
• species reactivity (predicted by homology): amphibian, monkey, rabbit, avian
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001019389
• UniProt accession no.: Q9NQX3
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... GPHN(10243)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Gephyrin. It targets an epitope within 25 amino acids from the C-terminal half of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human Gephyrin.

Application

Quality Control Testing

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gephyrin in Neuro-2a cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected Gephyrin in rat brain lysates and Myc/DDK-tagged recombinant human Gephyrin.

Enzyme Immunoassay (ELISA) Analysis: Serial dilutions from a representative lot detected Myc/DDK-tagged recombinant human Gephyrin.

Affinity Binding Assay Analysis: The binding affinity of this antibody to Gephyrin peptide was at least ten thousand-fold (10,000X) higher than that obtained with the non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Gephyrin (UniProt: Q9NQX3; also known as) is encoded by the GPHN (also known as) gene (Gene ID: 10243) in human. Gephyrin is a multifunctional enzyme and a multi-domain protein consisting of an N-terminal G-domain (Molybdopterin adenylyltransferase, EC:2.7.7.75), which interacts with receptors, a central linker region, and a C-terminal E-domain (Molybdopterin molybdenumtransferase, EC:2.10.1.1), which binds to microtubules. Gephyrin plays a critical role in the clustering and anchoring of inhibitory neurotransmitter receptors at the postsynaptic membrane of neurons. Its primary function is to cluster and localize glycine and gamma-aminobutyric acid (GABA) receptors at inhibitory synapses in the central nervous system. It interacts with these receptors through its PDZ domain, ensuring their proper positioning and functional integrity. Gephyrin is predominantly expressed in the brain and spinal cord, particularly in regions associated with inhibitory neurotransmission, such as the hippocampus and cerebellum. Its expression is crucial during brain development, contributing to synapse formation and maturation. Gephyrin can associate with microtubules in a subset of neuronal processes, potentially impacting neuronal morphology and intracellular transport. Mutations in the gene encoding gephyrin (GPHN) have been linked to certain types of neurodevelopmental disorders, including hyperekplexia and autism spectrum disorders. Hyperekplexia, also known as startle disease, is characterized by an exaggerated startle response, muscle stiffness, and an increased risk of sudden infant death syndrome (SIDS). These mutations disrupt gephyrin′s interaction with receptors, impairing inhibitory neurotransmission. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable