ZRB1656

Anti-p-TSC2 (Thr1462) Antibody, clone 1I17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Tuberin, Tuberous sclerosis 2 protein
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I17, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 200.60 kDa; observed mol wt ~200 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): rat, mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; inhibition assay: suitable (peptide); western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_000539
• UniProt accession no.: P49815
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TSC2(7249)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1I17 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects TSC2 phosphorylated on Threonine 1462.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal half of human Tuberin/TSC2.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK-293 cells treated with Calyculin A/Okadaic acid

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected p-TSC2 (Thr1462) in lysate from HEK-293 cells overnight serum starved and treated with 50 nM Calyculin A/500 nM Okadaic acid for 30 min.

Tested Applications

Peptide Inhibition Assay: Target band detection in lysate from HEK-293 cells overnight serum starved treated with 50 nM Calyculin A/500 nM Okadaic acid for 30 min was prevented by pre-blocking of a representative lot with the immunogen phosphorylated peptide, but not the corresponding non-phosphorylated peptide.

Affinity Binding Assay: A representative lot of this antibody bound p-TSC2 (Thr1462) peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Tuberin (UniProt: P49815; also known as Tuberous sclerosis 2 protein) is encoded by the TSC2 (also known as TSC4) gene (Gene ID: 7249) in human. TSC2 is a peripheral membrane protein that serves as a tumor suppressor and is expressed in multiple tissues. In complex with TSC1, it inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It acts as a GTPase-activating protein (GAP) for the small GTPase Rheb, a direct activator of the protein kinase activity of mTORC1. In its C-terminal region is a highly conserved Rap-Gap domain (aa 1531-1758) that is essential for its function. TSC2 has been reported to increase the intrinsic GTPase activity of both Rap1A and Rab5 in vitro. It can undergo phosphorylation at serine 1387, 1418, 1420 and 1798. However, these phosphorylations do not affect its interaction with TSC1. It can also undergo phosphorylation at serine 939 and threonine 1462 by Akt1 induced by growth factor stimulation. Phosphorylation of TSC2 at Thr1462 is primarily mediated by Akt, a serine/threonine-specific protein kinase that is activated in response to growth factors and insulin. This phosphorylation event inhibits the GTPase-activating protein (GAP) activity of the TSC1-TSC2 complex, leading to the activation of mTORC1 (mTOR complex 1) and promoting anabolic processes such as protein synthesis and lipid biosynthesis and inhibiting catabolic processes. Dysregulation of TSC2 phosphorylation, particularly at Thr1462, can lead to uncontrolled cell growth and is associated with diseases such as tuberous sclerosis complex, cancer, and metabolic disorders. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, Y., et al. (2004). Trends Biochem. Sci. 29(1); 32-38; Tee, AR., et al. (2002). Proc. Natl. Acad. Sci. USA. 99(21); 13571-13576

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable