Y3627

Yeast Carbon Base

suitable for microbiology, NutriSelect^® Basic

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | utilization test: suitable; microbiological culture: suitable
• application(s): clinical testing; food and beverages; life science and biopharma; microbiology
• storage temp.: 2-8°C
• suitability: nonselective for Candida spp.; nonselective for Pichia spp.; nonselective for Saccharomyces spp.; nonselective for Zygosaccharomyces spp.

Description

Application

Recommended for use in classification of yeasts on the basis of their ability to assimilate nitrogen compounds.

Components

Ingredients (g/L)
Dextrose, 10.00
L-Histidine hydrochloride, 0.001
DL-Methionine, 0.002
DL-Tryptophan, 0.002
Biotin, 0.000002
Calcium pantothenate, 0.0004
Folic acid, 0.000002
Inositol, 0.002
Niacin, 0.0004
p-Amino benzoic acid, 0.0002
Pyridoxine hydrochloride, 0.0004
Riboflavin (Vitamin B₂), 0.0002
Thiamine hydrochloride, 0.0004
Boric acid, 0.0005
Coppers sulfate, 0.00004
Potassium iodide, 0.0001
Ferric chloride, 0.0002
Manganese sulfate, 0.0004
Sodium molybdate, 0.0002
Zinc sulfate, 0.0004
Monopotassium phosphate, 1.00
Magnesium sulfate, 0.50
Sodium chloride, 0.10
Calcium chloride, 0.10

Preparation Note

A. Nitrogen Assimilation test
Prepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration.
B. Detection of yeasts, other than Saccharomyces cerevisae
Dissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water.
C. Detection of wild yeasts in beer and other brewing components
Add 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Gloves