SRP5038
HGK (1-328), active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687, MAP4K4, NIK
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
46-62 nmol/min·mg
mol wt
~64 kDa
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MAP4K4(9448)
General description
Hepatocyte progenitor kinase-like/germinal center kinase-like kinase (HGK) i or mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) is a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7(mitogen-activated protein kinase kinase kinase 7)/TAK1(transforming growth factor β-activated kinase 1), MAP2K4/MKK4 (mitogen-activated protein kinase kinase 4), and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-α signaling. HGK-dependent signaling inhibits peroxisome proliferator-activated receptor γ(PPARγ)-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport. MAP4K4 gene is mapped to human chromosome 2q11.2.
Biochem/physiol Actions
Mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) enzyme participates in promoting and suppressing inflammatory process. The MAP4K4 gene is upregulated in adenocarcinoma. Map4k4 in mice, is necessary for normal lymphatic vascular function. From endothelium-specific Map4k4 knockout models, it is found that atherosclerosis and lymphocyte recruitment might be induced by Map4k4. MAP4K4 is known to cause insulin resistance which is associated with Type 2 diabetes mellitus pathogenesis. It mediates tumor necrosis factor-α induced β cells necrosis.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
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MAP 4K4 is a novel MAPK/ERK pathway regulator required for lung adenocarcinoma maintenance.
Gao X, et al.
Molecular Oncology, 11(6), 628-639 (2017)
Association of common genetic variants in mitogen-activated protein kinase kinase kinase kinase 4 with type 2 diabetes mellitus in a Chinese Han population.
Li T T, et al.
Chinese Medical Journal (English Edition), 129(10), 1179-1179 (2016)
A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway.
Yao Z, et al.
The Journal of Biological Chemistry, 274(4), 2118-2125 (1999)
Endothelial MAP4K4 is critical for lymphatic vascular development and function.
Flach R J R, et al.
Molecular and Cellular Biology (2016)
Xiaoqing Tang et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(7), 2087-2092 (2006-02-08)
The insulin-regulated glucose transporter GLUT4 is a key modulator of whole body glucose homeostasis, and its selective loss in adipose tissue or skeletal muscle causes insulin resistance and diabetes. Here we report an RNA interference-based screen of protein kinases expressed
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