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SAB4200241

Sigma-Aldrich

Anti-PP2A subunit B isoform PR55-α antibody, Mouse monoclonal

clone 2G9, purified from hybridoma cell culture

Synonym(s):

Anti-B55α, Anti-PPP2R2A, Anti-PR52A, Anti-PR55A, Anti-Protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform, Anti-Protein phosphatase 2, regulatory subunit B, alpha, B55A, Anti-Serine/threonine protein phosphatase 2A, 55 kDa regulatory subunit B, alpha isoform

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2G9, monoclonal

form

buffered aqueous solution

mol wt

antigen 50-55 kDa

species reactivity

rat, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using whole extracts of mouse 3T3, rat NRK or human HeLa cells

isotype

IgG1

UniProt accession no.

P63151

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPP2R2A(5520)
mouse ... Ppp2r2a(71978)
rat ... Ppp2r2a(117104)

General description

Monoclonal Anti-PP2A subunit B isoform PR55- (mouse IgG1 isotype) is derived from the hybridoma 2G9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of rat PP2A subunit B isoform PR55-. PP2A subunit B isoform PR55- (PPP2R2A) belongs to the phosphatase (PP)2A regulatory subunit B family. Protein phosphatase 2A is one of the major Ser/Thr phosphatases. PPP2R2A encodes an alpha isoform of the regulatory subunit B55 subfamily. This gene is mapped to human chromosome 8p21.2.

Specificity

Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ.

Application

Monoclonal Anti-PP2A subunit B isoform PR55-α antibody produced in mouse has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates cellular invasion, localization of Sgo1 at centromeres, cell movement, Ras-1 activation, and chromosome segregation. PP2A protein complex consists of a dimeric core containing subunits A and C; which in turn associates with the regulatory subunit B. PP2A subunit B is involved in the degranulation of mast cells . Monoclonal Anti- PP2A subunit B isoform PR55-α binds to rat, human and mouse PP2A subunit B isoform PR55-α. The antibody may also react with rat PP2A-B55-δ, human PP2A-B55-β and weakly associates with rabbit PP2A-B55-γ.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Homozygous deletions and recurrent amplifications implicate new genes involved in prostate cancer
Liu W, et al.
Neoplasia, 10(8) (2008)
Sunanda Basu
Current protein & peptide science, 12(1), 3-11 (2010-12-31)
Cell motility is a very critical phenomenon that plays an important role in the development of eukaryotic organisms. One of the well studied cell motility phenomena is chemotaxis, which is described as a directional movement of cell in response to
Gregory Kranias et al.
Cellular signalling, 22(12), 1882-1890 (2010-08-07)
Asthma is characterised by antigen-mediated mast cell degranulation resulting in secretion of inflammatory mediators. Protein phosphatase 2A (PP2A) is a serine/threonine protein phosphatase composed of a catalytic (PP2A-C) subunit together with a core scaffold (PP2A-A) subunit and a variable, regulatory
Sean T DeGrande et al.
The Journal of biological chemistry, 288(2), 1032-1046 (2012-12-04)
Kinase/phosphatase balance governs cardiac excitability in health and disease. Although detailed mechanisms for cardiac kinase regulation are established, far less is known regarding cardiac protein phosphatase 2A (PP2A) regulation. This is largely due to the complexity of the PP2A holoenzyme
Zhanyun Tang et al.
Developmental cell, 10(5), 575-585 (2006-04-04)
Loss of sister-chromatid cohesion triggers chromosome segregation in mitosis and occurs through two mechanisms in vertebrate cells: (1) phosphorylation and removal of cohesin from chromosome arms by mitotic kinases, including Plk1, during prophase, and (2) cleavage of centromeric cohesin by
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