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RPMI-1640 Medium

AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Other Notes

Modified for autoclaving
Without L-glutamine and sodium bicarbonate.

Quantity

Formulated to contain 10.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhonghua Liu et al.
mBio, 12(3) (2021-05-20)
Inhibition of fungal growth by Congo red (CR) has been putatively associated with specific binding to β-1,3-glucans, which blocks cell wall polysaccharide synthesis. In this study, we searched for transcription factors (TFs) that regulate the response to CR and interrogated
Karla Lucía F Alvarez et al.
BMC veterinary research, 16(1), 230-230 (2020-07-08)
In the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells. However
Michael D Lewis et al.
The American journal of tropical medicine and hygiene, 81(6), 1041-1049 (2009-12-10)
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to
Sven D Willger et al.
PLoS pathogens, 4(11), e1000200-e1000200 (2008-11-08)
At the site of microbial infections, the significant influx of immune effector cells and the necrosis of tissue by the invading pathogen generate hypoxic microenvironments in which both the pathogen and host cells must survive. Currently, whether hypoxia adaptation is
Ryoichi Arita et al.
Diabetes, 58(1), 215-226 (2008-10-09)
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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