MAK485

Pyruvate Kinase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): PK Activity Assay Kit
• UNSPSC Code: 12352204
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): metabolic disorders; endocrinological disorders; cancer; hematological disorder
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Pyruvate Kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates.

Features and Benefits

• 0.1 to 50 U/L for colorimetric assay and 0.01 to 2 U/L for fluorometric assay

Suitability

The kit is suitable for pyruvate kinase activity determination in plasma, serum, and tissue samples, as well as for studying the effects of drugs on pyruvate kinase activity.

Principle

The Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 590 nm is directly proportional to the pyruvate generated by the pyruvate kinase in the sample.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3