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H0137

Sigma-Aldrich

HT Media Supplement (50×) Hybri-Max™

lyophilized powder, suitable for hybridoma, BioXtra

Synonym(s):

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

HT Media Supplement (50×) Hybri-Max™, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

Application

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max™ has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii
Gondim L, et al.
Experimental Parasitology, 163, 46-56 (2016)
Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell
Jiao A, et al.
International Journal of Endocrinology, 2018 (2018)
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress
Hashiguchi K, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 808, 10-19 (2018)
Kimberly A McCormack et al.
Parasitology research, 118(8), 2409-2417 (2019-06-15)
Human babesiosis, a tick-borne disease similar to malaria, is most often caused by the hemoprotozoans Babesia divergens in Europe, and Babesia microti and Babesia duncani in North America. Babesia microti is the best documented and causes more cases of human
Emmanuel Osei Mensah et al.
Journal of bioscience and bioengineering, 128(4), 487-494 (2019-04-30)
Since the use of protein therapeutics is effective for treating intractable human diseases, the production of biologic therapeutic agents has dramatically increased over the past three decades. The Chinese hamster ovary (CHO) cell lines are the most commonly used host
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Protocols

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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