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product name
BRL-1, 94121649
biological source
rat liver
growth mode
Adherent
karyotype
Diploid or pseudodiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat epithelial cells
Cell Line Description
Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.
Application
Feeder cells for co-culture with freshly isolated hepatocytes.
Culture Medium
Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculture Routine
Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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