C1374

CDK6/CyclinD1, active, His/GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): CCND1, Cell division protein kinase 6, BCL1, CDK6, D11S287E, MGC59692, PLSTIRE, PRAD1, U21B31
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 7.9-10.7 nmol/min·mg
• mol wt: CDK6 subunit ~40 kDa; cyclin D1 ~61 kDa
• technique(s): activity assay: suitable
• solubility: soluble; water: soluble
• UniProt accession no.: P24385; Q00534
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CCND1(595) , CDK6(1021)

Description

General description

Research area: APOPTOSIS

This recombinant product was expressed by baculovirus in Sf9 insect cells using an N-terminal His-tag on CDK6, and a GST-tag on Cyclin D1.

Application

CDK6/CyclinD1, active, His/GST tagged human has been used in: In vitro CDK6 activity assay to evaluate the effects of small-molecule inhibitors for therapeutic haematopoietic stem cells (HSC) expansion.

Biochem/physiol Actions

Cyclin Dependent Kinases6 (CDK6) is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. CDK6 kinase activity is detected during the mid-G1 phase of the cell cycle. It plays a crucial role in phosphorylating and regulating the activity of the retinoblastoma (RB), a tumor suppressor protein. Moreover, CDK6 has active involvement during transcription in hematopoietic stem cells (HSC) and tumor cells. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. The activation of cyclin D1-dependent kinase activity is found to be necessary to trigger the process of apoptosis.

Physical form

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable