MABT1333

Anti-pan-Actin Antibody, clone 2A3

clone 2A3, from mouse

• Synonym(s): Skeletal muscle alpha-actin, Smooth muscle alpha-actin, Cytoplasmic beta-actin, Cardiac muscle alpha-actin, Cytoplasmic gamma-actin, Smooth muscle gamma-actin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2A3, monoclonal
• species reactivity: human, rat, monkey, yeast, hamster, Drosophila, mouse
• packaging: antibody small pack of 25 μL
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001092
• UniProt accession no.: P60709
• target post-translational modification: unmodified
• Gene Information: human ... ACTA1(58) , ACTC1(70) , ACTG1(71)

Description

General description

Actin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments. Six different actin types have been reported. Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. This pan-actin antibody detects all isoforms actin.

Specificity

Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.

Application

Anti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Actins in HeLa cell lysate.

Target description

~42 kDa observed; 41.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.