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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6F9.2.12.1.5, monoclonal
mol wt
calculated mol wt 17.5 kDa
observed mol wt ~30 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
human ... IL17A(7314)
General description
Interleukin-17A (UniProt: Q16552; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the IL17A (also known as CTLA8, IL17) gene (Gene ID: 3605) in human. IL-17A is a proinflammatory cytokine secreted by activated CD4+ Th17 cells. It is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein. Its levels are up-regulated by IL23A-IL12B. IL-17 is reported to play an important role in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kB and co-stimulation of T cell proliferation. It is also involved in antimicrobial host defense and maintenance of tissue integrity. Its actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with (TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A also plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. In synergy with IL-17F, it is shown to mediate the production of antimicrobial b-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, which limits the entry of microbes through the epithelial barriers. (Ref.: Boisson, B., et al. (2013). Immunity. 39(4); 676-686; Wright, J.F., et al. (2008). J. Immunol. 181(4); 2799-2805).
Specificity
Clone 6F9.2.12.1.5 is a mouse monoclonal antibody that specifically detects human Interleukin-17A.
Immunogen
A recombinant fragment corresponding to amino acids 19 to 155 of human IL-17A.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant human IL-17A.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant human IL-17A.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-17A, clone 6F9.2.12.1.5, Cat. No. MABF3174, is a mouse monoclonal antibody that detects IL-17A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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