MAB4145

Anti-BCRP Antibody, clone BXP-34

culture supernatant, clone BXP-34, Chemicon^®

• Synonym(s): Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: BXP-34, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• isotype: IgG1
• suitability: not suitable for Western blot; not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_004827.2
• UniProt accession no.: Q9UNQ0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ABCG2(9429)

Description

General description

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.

Specificity

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

Immunogen

The Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.

Application

Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

Not suitable for paraffin-embedded tissues or for Western blotting

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Target description

70 kDa

Physical form

Liquid containing 0.7% BSA and 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.

Analysis Note

Control
MCF-7 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1