ABD53

Anti-ZEB1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• species reactivity (predicted by homology): frog (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), human (immunogen homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_110378
• UniProt accession no.: P37275
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ZEB1(6935)

Description

General description

Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8) and Negative regulator of IL2 or NIL-2-A zinc finger protein is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.

Immunogen

KLH-conjugated linear peptide corresponding to human ZEB1.

Application

Anti-ZEB1 detects levels of ZEB1 proteins & has been published & validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Neuroscience

Research Sub Category
Transcription Factors

Developmental Signaling

Quality

Evaluated by Western Blot in mouse embryo e11 tissue lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ZEB1 in 10 µg of mouse embryo e11 tissue lysate.

Target description

~200 kDa observed. The calculated molecular weight is 124 kDa, however ZEB1 has been shown at ~180-210 kDa in western blots (Dave, N., et al. (2011). J. Biol. Chem. 286(14):12024-12032).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse embryo e11 tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable