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AB5674P

Sigma-Aldrich

Anti-Acid Sensitive Ion Channel 1 Antibody

Chemicon®, from rabbit

Synonym(s):

ASIC1, BNC1, MDEG, BNaC1a, Amiloride-sensitive Cation Channel 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_001095.2
NM_020039.2

UniProt accession no.

P78348

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Recognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs.

Immunogen

A 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996).

The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2.

Application

Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL.

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 μg/mL on paraformaldehyde fixed tissue.

ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide (Catalog number AG394) per well.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neil D Detweiler et al.
Journal of applied physiology (Bethesda, Md. : 1985), 127(2), 393-407 (2019-06-07)
Acid-sensing ion channels (ASICs) are voltage-insensitive cation channels that contribute to cellular excitability. We previously reported that ASIC1 in pulmonary artery smooth muscle cells (PASMC) contribute to pulmonary vasoreactivity and vascular remodeling during the development of chronic hypoxia (CH)-induced pulmonary

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