8410-OP

SSPE Buffer

OmniPur^® Grade, 20X Liquid Concentrate

• Synonym(s): Saline-sodium Phosphate-EDTA Buffer, 20X
• UNSPSC Code: 41105319
• NACRES: NA.28

Properties

• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: clear colorless
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

General Composition: Each liter of concentrate makes 20 L of 1X SSPE buffer (1X = 0.150 M Sodium Chloride, 0.010 M Sodium Phosphate, 0.001 M EDTA). Molecular Biology grade.

DNase: None detected
pH of solution at 25°C: 7.25-7.55
Protease: None detected
RNase: None detected





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

• Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study examines the effects of various saline buffers, including SSPE, on the stability of gold nanoparticles used in biological and chemosensing applications. Findings indicate that SSPE buffer helps maintain nanoparticle stability, making it a valuable tool in bioengineering and sensing technologies (Zhou et al., 2020).


• Quantification of surface etching by common buffers and implications on the accuracy of label-free biological assays.: This research evaluates the impact of SSPE and other buffers on surface etching and its subsequent effect on the accuracy of label-free assays. The study highlights the importance of buffer selection in minimizing etching and improving assay reliability (Ahn et al., 2012).


• An optimized method for extraction and detection of Coconut cadang-cadang viroid(CCCVd) from oil palm.: This paper presents an optimized protocol using SSPE buffer for the extraction and detection of viroids from plant tissues. The method significantly enhances detection sensitivity, demonstrating SSPE buffer′s utility in plant virology research (Mohammadi et al., 2010).


• Common buffers, media, and stock solutions.: This comprehensive guide includes detailed protocols for preparing SSPE buffer, among other common laboratory solutions, emphasizing their roles in various molecular biology applications (Current Protocols in Human Genetics, 2001).


• Transcription inhibition and other properties of matrix proteins expressed by M genes cloned from measles viruses and diseased human brain tissue.: The study utilizes SSPE buffer in the analysis of viral matrix proteins from measles-infected brain tissues, highlighting its role in virological and neurological research (Suryanarayana et al., 1994).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable