R0781

Anti-Rab14 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-F protein-binding protein 1, Anti-GTPase Rab14, Anti-RAB14, member RAS oncogene family, Anti-Small GTP binding proein RAB14
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~24 kDa
• reaktywność gatunkowa: rat, human, mouse
• stężenie: ~1.0 mg/mL
• metody: indirect immunofluorescence: 2.5-5 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin; western blot: 2-4 μg/mL using whole extracts of mouse brain and human Raji cells
• numer dostępu UniProt: P61106
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... RAB14(51552) ; mouse ... Rab14(68365) ; rat ... Rab14(94197)

Opis

Opis ogólny

Ras-related protein Rab-14 (Rab14) is a member of the Rab11 subfamily of small guanosine triphosphatases (GTPases). It is associated with the early endosomal clathrin-coated trans‐Golgi network microdomain and is ubiquitously expressed. Rab14 belongs to the RAS oncogene family and is mapped to human chromosome 9q33.2.

Specyficzność

Anti-Rab14 recognizes human, rat, and mouse Rab14 (not tested in other species).

Zastosowanie

Anti-Rab14 antibody produced in rabbit has been used in western blotting and immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Działania biochem./fizjol.

Ras-related protein Rab-14 (Rab14) is associated with the trafficking of the fibroblast growth factor receptor 2 (FGFR2) and regulates N-cadherin turnover. It is involved in membrane trafficking between the Golgi complex and endosomes. In polarized epithelial cells, Rab 14 regulates delivery of cargo from the trans‐Golgi network (TGN) to the apical membrane domain. Rab14 has also been implicated in phagosomal biogenesis. It acts as a tumor oncogene in non-small cell lung carcinoma (NSCLC) and is upregulated in gastric cancer.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)