Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

View All Documentation

CS0390

Sigma-Aldrich

Mitochondria Staining Kit

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

Synonim(y):

JC-1 dye

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
12352207
NACRES:
NA.32

Poziom jakości

200

zastosowanie

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

opakowanie

pkg of 1 kit

warunki przechowywania

dry at room temperature

metody

flow cytometry: suitable
protein staining: suitable

fluorescencja

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

Zastosowanie

cell analysis
detection

metoda wykrywania

fluorometric

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

Zastosowanie

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

Cechy i korzyści

Kit offers:
  • A fast, simple, and convenient method for staining and assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
  • The shift in fluorescence is clearly detectable in both green and red channels
  • Suitable for adherent cells as well as cells in suspension
  • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
  • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis
  • DMSO 1 mL
  • JC Staining Buffer 5X 120 mL
  • JC-1 1 mg
  • Valinomycin Ready Made .1 mL

Kod klasy składowania

10 - Combustible liquids

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Klienci oglądali również te produkty

Slide 1 of 4

1 of 4

Mitochondria Isolation Kit sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sigma-Aldrich

MITOISO2

Mitochondria Isolation Kit

Rhodamine 123 mitochondrial specific fluorescent dye

Sigma-Aldrich

R8004

Rhodamine 123

Mitochondria Isolation Kit sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Sigma-Aldrich

MITOISO1

Mitochondria Isolation Kit

Anti-Mitochondria Antibody serum, Chemicon®

Sigma-Aldrich

AB3598

Anti-Mitochondria Antibody

Small Molecule Mediated Restoration of Mitochondrial Function Augments Anti-Mycobacterial Activity of Human Macrophages Subjected to Cholesterol Induced Asymptomatic Dyslipidemia.
Suman Asalla et al.
Frontiers in cellular and infection microbiology, 7, 439-439 (2017-10-27)
Robert L Elliott et al.
PloS one, 14(4), e0214586-e0214586 (2019-04-02)
Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life
Maria C Zanellati et al.
Frontiers in genetics, 6, 78-78 (2015-03-31)
Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed
Whasun Lim et al.
Journal of cellular physiology, 231(12), 2690-2699 (2016-03-13)
Apigenin is a flavonoid found in parsley, onions, oranges, tea, chamomile, wheat, and sprouts. It has a variety of biological properties including anti-oxidant, anti-mutagenic, anti-carcinogenic, anti-inflammatory, anti-proliferative, and anti-spasmodic effects. Based on epidemiological and case-control studies, apigenin is regarded as
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
Wyświetl wszystkie powiązane dokumenty

Produkty

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej