KARPAS-929

06072606, from human bone marrow

• Synonim(y): KARPAS 929, KARPAS, 929, KARPAS929
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human bone marrow
• tryb wzrostu: Suspension
• kariotyp: Hyperdiploid with complex aberrant chromosomes
• morfologia: Round to polygonal cells growing in clusters in suspension
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice

Opis

Pochodzenie linii komórkowej

Human multiple myeloma

Opis linii komórkowej

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

Profil DNA

Not specified

pożywka hodowlana

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rutyna subkultury

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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