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Merck

A9792

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonim(y):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

rabbit

Poziom jakości

białko sprzężone

peroxidase conjugate

forma przeciwciała

IgG fraction of antiserum

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

chicken

metody

direct ELISA: 1:30,000
dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate.
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

Specyficzność

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY.

Immunogen

purified chicken IgY (IgG)

Zastosowanie

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology.
Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays.

Działania biochem./fizjol.

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Uwaga dotycząca przygotowania

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Resp. Sens. 1 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Energetic stress, immunosuppression and the costs of an antibody response.
Svensson, E., et al.
Functional Ecology, 12(6), 912-919 (1998)
G Haqshenas et al.
The Journal of general virology, 83(Pt 9), 2201-2209 (2002-08-20)
We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis-splenomegaly (HS) syndrome in the USA. We showed that avian HEV is genetically related to swine and human HEVs. Here we report the antigenic
N Obermüller et al.
American journal of physiology. Renal physiology, 280(3), F540-F550 (2001-02-22)
The expansion of cysts in polycystic kidneys bears several similarities to the invasion of the extracellular matrix by benign tumors. We therefore hypothesized that cyst-lining epithelial cells produce extracellular matrix-degrading metalloproteinases and that the inhibition of these enzymes may represent
N Obermüller et al.
American journal of physiology. Renal physiology, 280(2), F244-F253 (2001-02-24)
Because proteinuria has been demonstrated in patients with autosomal-dominant polycystic kidney disease (ADPKD), we have investigated whether proteinuria also occurs in the (cy/+) rat, a widely used model for ADPKD. Increased urinary excretion of proteins, in particular of albumin, can
IgY: clues to the origins of modern antibodies
Warr G W, et al.
Immunology Today, 16(8), 392-398 (1995)

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