A4781
Asialofetuin from fetal calf serum
Type I (Sigma designation)
Synonim(y):
Asialofetuin
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine (calf) serum
Poziom jakości
typ
Type I (Sigma designation)
Postać
powder
zanieczyszczenia
salt, essentially free
≤0.5% N-acetylneuraminic acid
rozpuszczalność
0.85% sodium chloride: soluble 1 mg/mL
temp. przechowywania
2-8°C
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Opis ogólny
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.
Zastosowanie
Asialofetuin from fetal calf serum has been used:
- to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
- quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
- as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
- to study the nature of the interaction between ferritin and the placenta
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Działania biochem./fizjol.
Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.
Uwaga dotycząca przygotowania
Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Keiichi Motoyama et al.
Journal of drug delivery, 2011, 476137-476137 (2011-04-15)
The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed
R D Lamparelli et al.
British journal of haematology, 72(1), 100-105 (1989-05-01)
The organ distribution of intravenously injected hepatic ferritin either labelled with 59Fe or with 59Fe and 125I, was studied in pregnant guinea-pigs. At 5 h 71.2% of injected 59Fe was present in the placenta and fetus. Transfer of 59Fe to
Nida Zaidi et al.
International journal of biological macromolecules, 103, 65-73 (2017-05-13)
The SDS-glycoprotein system is mimic of membrane protein-lipid system. Fate of glycoprotein, conformation and the interactive forces involved in membrane milieu are expected to be decided by the net charge on glycoprotein that may change during acidic environment in a
Jakob Meier-Credo et al.
Journal of the American Society for Mass Spectrometry, 33(7), 1293-1302 (2022-06-28)
Identification and sequence determination by mass spectrometry have become routine analyses for soluble proteins. Membrane proteins, however, remain challenging targets due to their hydrophobicity and poor annotation. In particular small membrane proteins often remain unnoticed as they are largely inaccessible
Nak-Won Choi et al.
Journal of biotechnology, 121(2), 272-283 (2005-09-27)
The castor-oil plant Ricinus communis A-B dimeric toxin B subunit (RTB) was genetically linked at its N-terminus with a 90 amino acid peptide from simian rotavirus SA-11 non-structural protein NSP4(90) and produced in Escherichia coli BL21 cells. Biologically active recombinant
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