A4471
Anti-ARTS antibody, Mouse monoclonal
clone ARTS51, purified from hybridoma cell culture
Synonim(y):
Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway, Monoclonal Anti-ARTS antibody produced in mouse
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About This Item
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
ARTS51, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen 32 kDa
reaktywność gatunkowa
human
stężenie
~2 mg/mL
metody
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS
izotyp
IgG2b
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SEPT4(5414)
Opis ogólny
1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers.
Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.
Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule.
Specyficzność
This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)
This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.
This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.
Immunogen
synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.
Zastosowanie
Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.
Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Natalia Edison et al.
Cell reports, 21(2), 442-454 (2017-10-12)
We describe a mechanism by which the anti-apoptotic B cell lymphoma 2 (Bcl-2) protein is downregulated to induce apoptosis. ARTS (Sept4_i2) is a tumor suppressor protein that promotes cell death through specifically antagonizing XIAP (X-linked inhibitor of apoptosis). ARTS and
Kristi L Bennett et al.
Cancer research, 69(24), 9301-9305 (2009-11-26)
Head and neck squamous cell carcinoma (HNSCC) is an aggressive cancer with low survival rates in advanced stages. To facilitate timely diagnosis and improve outcome, early detection markers (e.g., DNA methylation) are crucial for timely cancer diagnosis. In a recent
A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif
Larisch S, et al.
Nature Cell Biology, 2(12), 915-915 (2000)
Dana Mamriev et al.
Cell death & disease, 11(6), 483-483 (2020-06-27)
Many human cancers over-express B cell lymphoma 2 (Bcl-2) or X-linked inhibitor of apoptosis (IAP) proteins to evade cell death. The pro-apoptotic ARTS (Sept4_i2) protein binds directly to both Bcl-2 and XIAP and promotes apoptosis by stimulating their degradation via
Ronit Elhasid et al.
Oncogene, 23(32), 5468-5475 (2004-05-04)
Acquired resistance towards apoptosis is the hallmark of most if not all types of cancer. We have previously identified and characterized ARTS, a broadly expressed protein localized to mitochondria. ARTS was initially shown to mediate TGF-beta induced apoptosis. Recently, we
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