MAB2575
Anti-Integrin α5β1 Antibody, clone BMC5
ascites fluid, clone BMC5, Chemicon®
Synonim(y):
VLA-5
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
BMC5, monoclonal
reaktywność gatunkowa
mouse
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
izotyp
IgG2b
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ITGA5(3678)
Specyficzność
Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Immunogen
Mouse embryonal carcinoma P19 cells.
Zastosowanie
Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.
Inhibition of cell adhesion to fibronectin: 10 μL/mL
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Postać fizyczna
Liquid ascites.
Przechowywanie i stabilność
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Invasion of eukaryotic cells by Borrelia burgdorferi requires ?(1) integrins and Src kinase activity.
Wu, J; Weening, EH; Faske, JB; Hook, M; Skare, JT
Infection and Immunity null
Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.
Legate, KR; Takahashi, S; Bonakdar, N; Fabry, B; Boettiger, D; Zent, R; Fassler, R
The Embo Journal null
Jessie Zhong et al.
PloS one, 7(4), e35058-e35058 (2012-04-18)
The speed of cell migration on 2-dimensional (2D) surfaces is determined by the rate of assembly and disassembly of clustered integrin receptors known as focal adhesions. Different modes of cell migration that have been described in 3D environments are distinguished
Mitasha Bharadwaj et al.
Nature communications, 8, 14348-14348 (2017-01-28)
Upon binding to the extracellular matrix protein, fibronectin, αV-class and α5β1 integrins trigger the recruitment of large protein assemblies and strengthen cell adhesion. Both integrin classes have been functionally specified, however their specific roles in immediate phases of cell attachment
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