ABE1934

Anti-Gcn5 Antibody

from rabbit

• Synonim(y): Histone acetyltransferase KAT2A, General control of amino acid synthesis protein 5-like 2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: mouse, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology)
• metody: ChIP: suitable (ChIP-seq); immunoprecipitation (IP): suitable; western blot: suitable
• numer dostępu NCBI: NP_066564
• numer dostępu UniProt: Q92830
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... KAT2A(2648)

Opis

Opis ogólny

Histone acetyltransferase KAT2A (EC 2.3.1.48; UniProt Q92830; also known as General control of amino acid synthesis protein 5-like 2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID 2648) in human. Histone acetyltransferases (HATs) are transcription co-activators recruited by transcriptional activators to target gene promoters, where they enhance the transcription activity by acetylating lysine residues within the N-terminal regions of histones. KAT2A/KAT2B, MYST (MOZ, Ybf2 (Sas3), Sas2, and Tip60), TAFII250, CREBBP/EP300, and SRC represent the five HAT families. In addtion to functioning as transcription co-activators, KAT2A (GCN5) and KAT2B (PCAF) are also reported to repress IFN‐β production and innate antiviral immunity by inhibiting the innate immune signaling kinase TBK1 in the cytoplasm in a HAT‐independent and non‐transcriptional manner.

Specyficzność

This polyclonal antibody was raised against human KAT2A isoform GCN5-L N-terminal sequence that is 95% conserved in murine and rat Kat2a/Gcn5, but missing in human spliced isoform GCN5-S (UniProt Q92830-2).

Immunogen

Epitope: N-terminus.

Ovalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human Gcn5.

Zastosowanie

Anti-Gcn5 Antibody is an antibody against Gcn5 for use in Immunoprecipitation, Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Western Blotting Analysis: 1.2 µg/mL from a representative lot detected Gcn5 immunoprecipited from HeLa nuclear extract.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immunoprecipitated KAT2A/GCN5-associated DNA fragments by ChIP using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).
ChIP-seq Analysis: A representative lot was employed to generate Gcn5 genome-wide binding profiles by ChIP-seq analysis using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).

Jakość

Evaluated by Immunoprecipitation in HeLa nuclear extract.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipited Gcn5 from 500 µg of HeLa nuclear extract.

Opis wartości docelowych

~100 kDa observed. 93.93 kDa (human GCN5-L; UniProt Q92830-1) calculated.

Postać fizyczna

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable