648310-M

Tris Base, Molecular Biology Grade

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• Synonim(y): Tris(hydroxymethyl)aminomethane, tris(Hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
• Wzór liniowy: NH₂C(CH₂OH)₃
• Numer CAS: 77-86-1
• Masa cząsteczkowa: 121.14
• Beilstein: 741883
• Numer MDL: MFCD00004679
• Kod UNSPSC: 12161705

Właściwości

• product name: Tris Base, Molecular Biology Grade, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• klasa czystości: Molecular Biology
• Poziom jakości: 100
• Próba: ≥99% (perchloric acid titration)
• Postać: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• kolor: white
• przydatny zakres pH: 7-9
• pKa (25°C): 8.1
• tw: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• rozpuszczalność: water: 500 mg/mL
• ślady kationów: heavy metals: ≤0.0005%
• absorpcja: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• obecność zanieczyszczeń: RNases, DNases, proteases, none detected
• temp. przechowywania: 10-30°C
• ciąg SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Klucz InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Opis

Opis ogólny

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Zastosowanie

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Ostrzeżenie

Toxicity: Irritant (B)

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable