251515-M

DAF-FM

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Synonim(y): DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
• Wzór empiryczny (zapis Hilla): C₂₁H₁₄F₂N₂O₅
• Numer CAS: 254109-20-1
• Masa cząsteczkowa: 412.34
• Kod UNSPSC: 12352200

Właściwości

• Poziom jakości: 100
• Próba: ≥98% (HPLC)
• Postać: solution
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: pale yellow
• fluorescencja: λ_ex ~500 nm; λ_em ~515 nm
• temp. przechowywania: −20°C
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• Klucz InChI: DIJCILWNOLHJCG-UHFFFAOYSA-N

Opis

Opis ogólny

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8.

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Działania biochem./fizjol.

Primary Target
Nitric oxide (NO) fluorescent indicator

Ostrzeżenie

Toxicity: Irritant (B)

Postać fizyczna

In DMSO.

Rekonstytucja

Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.

Inne uwagi

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.

Informacje prawne

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): 188.6 °F
• Temperatura zapłonu (°C): 87 °C