LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18
CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] water; [B] acetonitrile; (5:95, A:B)
flow rate
0.4 mL/min
column temp.
40 °C
detector
MS, ESI+
injection
2 μL
sample
1 μg/mL in acetonitrile
Description
Analysis Note
Analysis was conducted using standard solution, detection on LC/MS system.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany