LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis^® Express C18 at Elevated Temperature and Flow

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)

flow rate

0.6 mL/min

pressure

5440 psi (375 bar)

column temp.

35 °C

detector

ESI(+), TOF

injection

1 μL

sample

20 μg/mL in 0.1% formic acid