Skip to Content
Merck
  • Change in chemical constituents and free radical-scavenging activity during Pear (Pyrus pyrifolia) cultivar fruit development.

Change in chemical constituents and free radical-scavenging activity during Pear (Pyrus pyrifolia) cultivar fruit development.

Bioscience, biotechnology, and biochemistry (2014-10-29)
Jeong-Yong Cho, Sang-Hyun Lee, Eun Hee Kim, Hae Rim Yun, Hang Yeon Jeong, Yu Geon Lee, Wol-Soo Kim, Jae-Hak Moon
ABSTRACT

Changes in chemical constituent contents and DPPH radical-scavenging activity in fruits of pear (Pyrus pyrifolia) cultivars during the development were investigated. The fruits of seven cultivars (cv. Niitaka, Chuhwangbae, Wonhwang, Hwangkeumbae, Hwasan, Manpungbae, and Imamuraaki) were collected at 15-day intervals after day 20 of florescence. Vitamins (ascorbic acid and α-tocopherol), arbutin, chlorogenic acid, malaxinic acid, total caffeic acid, total flavonoids, and total phenolics were the highest in immature pear fruit on day 20 after florescence among samples at different growth stages. All of these compounds decreased gradually in the fruit during the development. Immature pear fruit on day 35 or 50 after florescence exhibited higher free radical-scavenging activity than that at other times, although activities were slightly different among cultivars. The chemical constituent contents and free radical-scavenging activity were largely different among immature fruits of the pear cultivars, but small differences were observed when they matured.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium carbonate-12C, 99.9 atom % 12C
Sigma-Aldrich
Ethyl acetate, ≥99%, FCC, FG
Sigma-Aldrich
Ethyl acetate, natural, ≥99%, FCC, FG
Supelco
Phenol solution, 5000 μg/mL in methanol, certified reference material
Supelco
Sodium carbonate concentrate, 0.1 M Na2CO3 in water, eluent concentrate for IC
Millipore
Bifido Selective Supplement B, suitable for microbiology
Supelco
5α-Androstan-17β-ol-3-one, VETRANAL®, analytical standard
Supelco
Arbutin, analytical standard
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Supelco
Gallic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
Phenol solution, certified reference material, 500 μg/mL in methanol
Supelco
Phenol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
2,2-Diphenyl-1-picrylhydrazyl
Sigma-Aldrich
Arbutin, ≥98% (HPLC)
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, ≥97.5%
Sigma-Aldrich
Phenol, ≥99%
Sigma-Aldrich
1,1-Diphenyl-2-picrylhydrazine, 97%
Sigma-Aldrich
Ethyl acetate, ReagentPlus®, ≥99.8%
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Supelco
Ethyl acetate, analytical standard
Arbutin, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Phenol, natural, 97%, FG
Supelco
Ethyl Acetate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Ethyl acetate
Sigma-Aldrich
Ultrapure Acetonitrile
Sigma-Aldrich
Ethyl acetate