- On the mechanism of background silver staining during sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
On the mechanism of background silver staining during sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
Electrophoresis (1999-08-18)
G Patras, G G Qiao, D H Solomon
PMID10451112
ABSTRACT
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox initiator system using ammonium persulphate (APS) and an amine such as N,N,N',N'-tetramethylethylenediamine (TEMED).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
N,N,N′,N′-Tetramethylethylenediamine, BioReagent, for molecular biology, ≥99% (GC)