- A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies.
A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies.
Lab on a chip (2013-02-16)
Lucía D Garza-García, Leydi M Carrillo-Cocom, Diana Araiz-Hernández, Pedro Soto-Vázquez, Julián López-Meza, Eduardo J Tapia-Mejía, Sergio Camacho-León, Erika García-López, Ciro A Rodríguez-González, Mario M Alvarez
PMID23412111
ABSTRACT
We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivities 100 times higher than those observed in full scale systems.
MATERIALS
Product Number
Brand
Product Description
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, average Mw 97,000 (Typical), average Mn 46,000 (Typical)