Skip to Content
Merck
  • Isoniazid loaded core shell nanoparticles derived from PLGA-PEG-PLGA tri-block copolymers: in vitro and in vivo drug release.

Isoniazid loaded core shell nanoparticles derived from PLGA-PEG-PLGA tri-block copolymers: in vitro and in vivo drug release.

Colloids and surfaces. B, Biointerfaces (2013-01-10)
Mani Gajendiran, Venkatachalam Gopi, Vellaichamy Elangovan, Rajagopalan Venkatakrishna Murali, Sengottuvelan Balasubramanian
PMID23298594
ABSTRACT

A series of biodegradable low molecular weight PLGA-PEG-PLGA tri-block copolymers have been synthesized in powder form. The anti-tuberculosis drug Isoniazid (INH) loaded polymeric core-shell nanoparticles (CSNPs) have been prepared by sonication followed by water-in-oil-in-water (w/o/w) double emulsification technique. The nanoparticles (NPs) have been characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), powder X-ray diffraction (XRD) and X-ray photo electron spectroscopic (XPS) techniques. The drug loaded CSNPs were found to be 150-400 nm in size with spherical shape. The drug loading efficiency and drug content of the polymer NPs were determined by UV-vis spectrophotometry. The drug loading efficiency and drug content of the NPs were (12.8-18.67%) and (6.4-8.9%) respectively. The in vitro release behavior of the polymer NPs has been investigated by UV-vis spectrophotometry and the release kinetics mechanism has been evaluated by Korsemeyer-Peppas (KP) and Higuchi models. The in vitro release studies show initial burst release followed by controlled and uniform release for longer duration. The pharmacokinetic studies show that the INH bioavailability of INH loaded CSNPs is 28 fold higher than that of free INH and also the CSNPs show sustained drug release for longer duration.

MATERIALS
Product Number
Brand
Product Description
P2066
Sigma-Aldrich
Poly(D,L-lactide-co-glycolide), lactide:glycolide 65:35, Mw 40,000-75,000
P1941
Sigma-Aldrich
Poly(D,L-lactide-co-glycolide), lactide:glycolide (75:25), mol wt 66,000-107,000
P2191
Sigma-Aldrich
Poly(D,L-lactide-co-glycolide), lactide:glycolide (50:50), mol wt 30,000-60,000
430471
Sigma-Aldrich
Poly(D,L-lactide-co-glycolide), ester terminated, Mw 50,000-75,000
719897
Sigma-Aldrich
Resomer® RG 502 H, Poly(D,L-lactide-co-glycolide), acid terminated, viscosity 0.16-0.24 dL/g 
719862
Sigma-Aldrich
Resomer® RG 653 H, Poly(D,L-lactide-co-glycolide), acid terminated, Mw 24,000-38,000
719889
Sigma-Aldrich
Resomer® RG 502, Poly(D,L-Lactide-co-Glycolide), lactide:glycolide 50:50, ester terminated, Mw 7,000-17,000
719870
Sigma-Aldrich
Resomer® RG 503 H, Poly(D,L-lactide-co-glycolide), acid terminated, lactide:glycolide 50:50, Mw 24,000-38,000
719927
Sigma-Aldrich
Resomer® RG 756 S, Poly(D,L-lactide-co-glycolide), ester terminated, lactide:glycolide 75:25, Mw 76,000-115,000
719919
Sigma-Aldrich
Resomer® RG 752 H, Poly(D,L-lactide-co-glycolide), acid terminated, lactide:glycolide 75:25, Mw 4,000-15,000
739952
Sigma-Aldrich
Resomer® RG 503, Poly(D,L-lactide-co-glycolide), lactide:glycolide 50:50, ester terminated, Mw 24,000-38,000
739979
Sigma-Aldrich
Resomer® RG 858 S, Poly(D,L-lactide-co-glycolide), ester terminated, lactide:glycolide 85:15, Mw 190,000-240,000
739944
Sigma-Aldrich
Resomer® RG 504, Poly(D,L-lactide-co-glycolide), lactide:glycolide 50:50, ester terminated, Mw 38,000-54,000
719900
Sigma-Aldrich
Resomer® RG 504 H, Poly(D,L-lactide-co-glycolide), acid terminated, lactide:glycolide 50:50, Mw 38,000-54,000
739960
Sigma-Aldrich
Resomer® RG 505, Poly(D,L-lactide-co-glycolide), ester terminated, Mw 54,000-69,000