Skip to Content
Merck
HomeProductsChemistry & BiochemistryBiochemistryBiological Buffers

Biological Buffers

Image of light blue biological buffer solution in beaker with pipette.

We understand the importance of protecting the integrity of your biomolecules and reagents with the right buffering systems. Our biological buffers provide solution stability and pH control without interfering with biological processes, and supply critical salts and nutrients for cells and tissues. Our advanced buffering systems can bring you phenomenal stability in cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation applications. All of our products can be scaled from early research to commercial applications in varying buffer grades. We offer numerous packaging configurations, custom blending, and even liquid formulation.

Complete Workflow Solutions

We also supply a comprehensive selection of high-quality biochemicals in a practical range of grades and in a variety of innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing, blending and packaging to meet your needs.

Related Product Literature
Buffer Reference Center
These tables will help you navigate the preparation of many common buffer solutions by pH and pKa. Choose a buffer with an effective pH range for your specific assay.
TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
M-Clarity™ Program
The MQ levels of the M-Clarity™ Program provide transparency so that you can choose, with confidence, suitable products that meet your needs.
Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Bioperformance Certified Buffers

In order to meet your buffer management challenges, opt for buffer quality rather than quantity. Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocess workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include testing for nuclease, protease, and phosphatase enzymatic activity, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures experiment reproducibility and reduces time, resource costs and even laboratory space – so that you can get on with your research endeavors.

pHast Pack™ Ready-to-Use Buffers

Trying to calibrate the correct concentration and pH value in buffer preparation can be a tedious, time-consuming task for scientists. Our pHast Pack™ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick results that are reliably reproducible again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.

Need help making a buffer? Our buffer calculator helps you to quickly calculate buffer molarity, mass, and volume.

Related Product Categories
Cell Culture & Analysis
Comprehensive cell culture workflow tools and advanced cell culture solutions from cell culture media to sera, supplements, cultureware, primary cultures, and established cell lines for basic research, drug discovery, and manufacturing.
Molecular Biology
Molecular Biology
PCR Reagents & Kits
PCR Reagents & Kits
Protein Electrophoresis & Western Blotting
Browse our products for protein electrophoresis and Western blotting, including Immobilon transfer membranes, transfer buffers, electrophoresis equipment, gels, gel stains, protein markers, and immunodetection reagents.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Detergents
We offer a broad range of biological detergents and surfactants, including REACH-compliant biodegradable alternatives, for use in cell lysis, DNA/RNA electrophoresis, Western blotting, transfection, and many other life science research applications.
Antibiotics
We offer a comprehensive portfolio of antibiotics to prevent contamination of cell cultures through pathogens or other cell lines, for genetic marker selection and cell biology research.
Related Applications
IVD Manufacturing
A brief overview on the key components involved in successful In vitro diagnostic (IVD) assay development. Learn more on IVD scale up, manufacturing, and commercialization.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Quantitation
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Related Services
Bulk & Custom Packaging
Bulk & Custom Packaging

Highlights

Slide 1 of 2
Redi-Dri™ – The world of free-flowing hygroscopic products
Redi-Dri™ – The world of free-flowing hygroscopic products
The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?