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Roche

Interleukin-2, mouse (mIL-2)

recombinant (E. coli)

Synonym(s):

mIL-2, mouse interleukin-2

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About This Item

UNSPSC Code:
12352207

biological source

mouse

Quality Level

100

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

solution

potency

<0.5 ng/mL EC50

mol wt

17,200 Da

packaging

pkg of 10,000 U (5 μg, 1 ml)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

technique(s)

inhibition assay: suitable

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

P04351

storage temp.

−20°C

Gene Information

mouse ... Il2(16183)

General description

Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).
Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.

Specificity

Mouse IL-2 is effective on mouse cells, but not on human cells.

Application

Recombinant murine IL-2 is produced in E. coli and exhibits the following:
  • Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
  • Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
  • Weakly inhibits the binding to human responder cells.
  • Shares identical biological and immunological activities with human IL-2.
  • Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
  • used for the stimulation of murine T-cells

Quality

Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequence

Chain Length 149 AA
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).

Preparation Note

Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.

Other Notes

Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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