RMHE-120K

MILLIPLEX^® Rat Metabolic Hormone Expanded Panel - Metabolism Multiplex Assay

Properties

• usage: kit sufficient for 96 wells
• Quality Level: 200
• species reactivity: rat
• manufacturer/tradename: Milliplex^®
• assay range: standard curve range: 1.4-1,000 pg/mL; (TNFα); standard curve range: 110-80,000 pg/mL; (IL-6); standard curve range: 110-80,000 pg/mL; (Insulin); standard curve range: 2.7-2,000 pg/mL; (Glucagon); standard curve range: 27-20,000 pg/mL; (GLP-1 (active)); standard curve range: 27-20,000 pg/mL; (GLP-1 total); standard curve range: 4.1-3,000 pg/mL; (GIP (total)); standard curve range: 4.1-3,000 pg/mL; (Ghrelin (active)); standard curve range: 41-30,000 pg/mL; (Amylin (active)); standard curve range: 48-35,000 pg/mL; (Leptin); standard curve range: 6.9-5,000 pg/mL; (PYY total); standard curve range: 6.9-5,000 pg/mL; (Pancreatic Polypeptide, PP); standard curve range: 6.9-5,000 pg/mL; (Secretin); standard curve range: 69-50,000 pg/mL; (C-Peptide 2); standard curve range: 69-50,000 pg/mL; (MCP-1/CCL2)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex^® xMAP^®)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

MILLIPLEX^® Rat Metabolic Hormone Expanded Panel is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/lysate and culture supernatant samples and rat serum or plasma: Active Amylin, C-peptide 2, Active Ghrelin, Gastric Inhibitory Polypeptide (GIP), Glucagon-like peptide-1 (GLP-1) Active or Total, Glucagon, Interleukin-6 (IL-6), Insulin, Leptin, Monocyte Chemoattractant Protein-1 (MCP-1), Pancreatic Polypeptide (PP), Peptide YY (PYY), Secretin, and Tumor Necrosis Factor alpha (TNFα). Notes: GLP-1 (Active) and GLP-1 (Total) cannot be run together in the same assay. Protease inhibitors should be added upon collection of samples.

Research Category: Metabolism & Endocrinology

Research Sub-Category:

• Metabolic Syndrome
• Obesity
• Diabetes

Application

The MILLIPLEX^® Rat Metabolic Hormone Expanded Panel is a bead-based quantitative assay based on the Luminex^® xMAP^® technology, which enables the simultaneous analysis of up to 14 analytes in a 25 µl sample volume. This is an overnight assay requiring 25 µL rat serum, plasma, or cell/tissue culture supernatants or lysates. No sample dilution is required.

Analytes Available: Amylin (active);C-Peptide 2; Ghrelin (active);GIP (total);GLP-1 (active);GLP-1 (total);Glucagon;IL-6;Insulin;Leptin;MCP-1/CCL2;Pancreatic Polypeptide (PP);PYY (total);Secretin;TNFα.

Cross Reactivity: There was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.

Sensitivity: Refer to kit protocol for sensitivities of each analyte.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332 - H311 - H315 - H317 - H319 - H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3