MAB2079Z
Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free
clone HUTS-4, Chemicon®, from mouse
Synonym(s):
Ligand-inducedBindingSite, LIBS, CLIBS, MAB2079
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HUTS-4, monoclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
rat ... Itgb1(24511)
General description
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Specificity
Monoclonal antibody MAB2079Z is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as MAB2079Z which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody MAB2079Z recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.
Immunogen
Epitope: activated, azide free
Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. MAB2079Z was specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
Application
Detect Integrin β1 using this Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free validated for use in ELISA, FC, IH and WB.
ELISA
Immunohistochemistry: fresh frozen, or light fixation, with 4% PFA permeabilized with 0.1% triton X-100 in the presence of Mn++ as the epitope of HUTS-4 is dependent upon Mn++ ion. Note antibody only stains activated beta1 integrins. Activated beta-1 integrins are typically found in wound regions for instance (see http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=eurekah.figgrp.12951) . Use MAB1981 or CBL1333 for general beta1 integrin staining and detection.
Western Blotting: non-reducing conditions only (see: http://www.jbc.org/cgi/content/full/271/19/11067).
FACS analysis: 2.5 μg antibody / 5 x 105 cells.
Immunohistochemistry: fresh frozen, or light fixation, with 4% PFA permeabilized with 0.1% triton X-100 in the presence of Mn++ as the epitope of HUTS-4 is dependent upon Mn++ ion. Note antibody only stains activated beta1 integrins. Activated beta-1 integrins are typically found in wound regions for instance (see http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=eurekah.figgrp.12951) . Use MAB1981 or CBL1333 for general beta1 integrin staining and detection.
Western Blotting: non-reducing conditions only (see: http://www.jbc.org/cgi/content/full/271/19/11067).
FACS analysis: 2.5 μg antibody / 5 x 105 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Target description
88 kDa
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in PBS containing no preservatives.
Protein A purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Human lung, liver, and skeletal muscle tissues
Human lung, liver, and skeletal muscle tissues
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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