Tools for improving yields and purity from recombinant bacterial proteins

WEBINAR

ABSTRACT

Various methods for the extraction and purification of recombinant proteins from bacteria are in use and have been well described. Common methods for cell lysis involve mechanical disaggregation of cells, such as sonication, but this can be time-consuming when working with multiple samples and more importantly can result in localized heating leading to loss of protein activity. In this webinar, we provide data showing that gentle detergent based lysis leads to greater protein activity and higher yields when compared to mechanical methods such as sonication. We also investigate the use of dual purification strategies and explore optimizing conditions to improve the purity of protein purification techniques.

WHAT WILL YOU LEARN?

• Optimal detergent selection to maximize yield of target protein of interest
  
• Detergent choice and how it impacts detergent removal for downstream applications
  
• Simple protein purification strategies to improve yield & quality
  
• Choosing between dialysis and diafiltration for sample clean-up and buffer exchange

WHO SHOULD WATCH?

Life science researchers who need to extract, isolate and purify recombinantly expressed proteins and preserve proteins while performing buffer exchange