HPLC Analysis of Dopamine and Related Compounds on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium formate (pH 3.0 with concentrated formic acid), [B] acetonitrile

flow rate

1 mL/min, split to the MS

column temp.

35 °C

detector

MS in SIR (Selected Ion Recording) mode using (+) or (-) ESI mode

injection

25 μL

sample

50 μg/mL in 10 mM ammonium formate (pH 3.0 with formic acid)