HPLC Analysis of Warfarin Enantiomers on Astec^® CHIROBIOTIC^® V (Method 2)

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 0.1% triethylamine acetate, pH 5.0; [B] THF; (80:20, A:B)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

1 μL

sample

4 mg/mL in methanol