HPLC Analysis of Taxols on Ascentis® Express F5
CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase
[A] water; [B] acetonitrile; (60:40, A:B)
flow rate
0.3 mL/min
column temp.
30 °C
detector
UV, 227 nm
injection
2 μL
sample
25 mg/L in 70:30, water:methanol
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of taxols.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany