HPLC Analysis of Histidine Enantiomers on Astec^® CHIROBIOTIC^® T (15 °C)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.006, A:B:C)

flow rate

0.5 mL/min

column temp.

15 °C

detector

UV, 205 nm

injection

10 μL

sample

200 μg /mL in 50:50 water:methanol