RAS GTPases are modified by SUMOylation.

RAS GTPases are modified by SUMOylation.

Oncotarget (2018-02-13)

Byeong Hyeok Choi, Changyan Chen, Mark Philips, Wei Dai

ABSTRACT

RAS proteins are GTPases that participate in multiple signal cascades, regulating crucial cellular processes including cell survival, proliferation, differentiation, and autophagy. Mutations or deregulated activities of RAS are frequently the driving force for oncogenic transformation and tumorigenesis. Given the important roles of the small ubiquitin-related modifier (SUMO) pathway in controlling the stability, activity, or subcellular localization of key cellular regulators, we investigated here whether RAS proteins are posttranslationally modified (

MATERIALS

Product Number

Brand

Product Description

P8340

Sigma-Aldrich

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

L6876

Sigma-Aldrich

Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

E1014

Millipore

Benzonase^® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution