- Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.
Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.
Scientific reports (2017-06-21)
R Sartaj, C Zhang, P Wan, Z Pasha, V Guaiquil, A Liu, J Liu, Y Luo, E Fuchs, M I Rosenblatt
PMID28630424
ABSTRACT
In order to identify reliable markers of corneal epithelial stem cells, we employed an inducible transgenic "pulse-chase" murine model (K5Tta × TRE-H2BGFP) to localize, purify, and characterize slow cycling cells in the cornea. The retention of GFP labeling in slowly dividing cells allowed for localization of these cells to the corneal limbus and their subsequent purification by FACS. Transcriptome analysis from slow cycling cells identified differentially expressed genes when comparing to GFP
MATERIALS
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Product Description
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Anti-BrdU Antibody, clone PRB-1, biotin conjugated, clone PRB-1, Chemicon®, from mouse