Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

H1758

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology

T2885

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

L4777

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Lipase acrylic resin, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

860336

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3,3′,5,5′-Tetramethylbenzidine, ≥99%

322415

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Methanol, anhydrous, 99.8%

439126

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Acetone, suitable for HPLC, ≥99.9%

W332607

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Acetone, ≥99%, meets FCC analytical specifications

T0440

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3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate

2104

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

717975

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W530574

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Hydrochloric acid solution, 32 wt. % in H₂O, FCC

T0167

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D-(+)-Trehalose dihydrate, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

T9531

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥99% (HPLC), from Saccharomyces cerevisiae

642991

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S7388

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439223

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Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

276855

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Dimethyl sulfoxide, anhydrous, ≥99.9%

D2650

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Dimethyl sulfoxide, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

606723

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270997

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Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

W241407

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Ethyl acetate, ≥99%, FCC, FG

W241415

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Ethyl acetate, natural, ≥99%, FCC, FG

D8418

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D2438

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D1435

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C5670

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