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  • Numb-deficient satellite cells have regeneration and proliferation defects.

Numb-deficient satellite cells have regeneration and proliferation defects.

Proceedings of the National Academy of Sciences of the United States of America (2013-10-31)
Rajani M George, Stefano Biressi, Brian J Beres, Erik Rogers, Amanda K Mulia, Ronald E Allen, Alan Rawls, Thomas A Rando, Jeanne Wilson-Rawls
ABSTRACT

The adaptor protein Numb has been implicated in the switch between cell proliferation and differentiation made by satellite cells during muscle repair. Using two genetic approaches to ablate Numb, we determined that, in its absence, muscle regeneration in response to injury was impaired. Single myofiber cultures demonstrated a lack of satellite cell proliferation in the absence of Numb, and the proliferation defect was confirmed in satellite cell cultures. Quantitative RT-PCR from Numb-deficient satellite cells demonstrated highly up-regulated expression of p21 and Myostatin, both inhibitors of myoblast proliferation. Transfection with Myostatin-specific siRNA rescued the proliferation defect of Numb-deficient satellite cells. Furthermore, overexpression of Numb in satellite cells inhibited Myostatin expression. These data indicate a unique function for Numb during the initial activation and proliferation of satellite cells in response to muscle injury.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Barium chloride dihydrate, ACS reagent, ≥99%
Sigma-Aldrich
Barium chloride, 99.999% trace metals basis
Sigma-Aldrich
Barium chloride, 99.9% trace metals basis