- A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.
A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.
STAR protocols (2021-11-11)
Walter E Knight, Yingqiong Cao, Phoebe Dillon, Kunhua Song
PMID34755117
ABSTRACT
When cultured under typical conditions, human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are structurally and functionally immature. We have previously demonstrated that culture of hiPSC-CMs in maturation medium containing fatty acids, in combination with culture on micropatterned surfaces, produces cells that demonstrate a more mature phenotype compared to standard approaches. Here, we show in detail the steps needed to produce mature hiPSC-CMs. Compared with many approaches, our protocol is relatively simple and can be easily adapted to new laboratories. For complete details on the use and execution of this protocol, please refer to Knight et al. (2021).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution