- Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Journal of bacteriology (2003-11-18)
Masayuki Kagawa, Zui Fujimoto, Mitsuru Momma, Kenji Takase, Hiroshi Mizuno
PMID14617662
ABSTRACT
The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process.