GE17-5157-01

HiTrap^® SP Fast Flow

Cytiva 17-5157-01, pack of 5 × 5 mL

• Synonym(s): HiTrap SP
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: sulphopropyl
• description: Ion Exchanger Type (value)
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 17-5157-01
• parameter: flow rate; 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 3-14
• working range: 4-13
• suitability: suitable for bioprocess medium

Description

General description

HiTrap^® SP FF is prepacked with SP Sepharose^™ Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose^™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Features and Benefits

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
• The industry standard for ion exchange chromatography.
• High flow rates and good scale-up potential.
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Predictable scale-up

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)

Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids