52595

Atto 514

suitable for fluorescence

• Synonym(s): Atto 514 carboxy, Atto 514 free acid
• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• Assay: ≥90.0% (HPLC)
• Quality Level: 100
• form: solid
• mol wt: M_w 868 g/mol
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm; 514 nm
• fluorescence: λ_ex 511 nm; λ_em 533 nm±10 nm in PBS, pH 7.4
• λ: (in water/methanol 1:1 with 0.1% perchloric acid)
• UV absorption: λ: 511-517 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield and exceptional thermal and photo-stability. Thus Atto 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of the Argon-Ion laser.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable