SITRAN-RO
Roche
X-tremeGENE™ siRNA Transfection Reagent
Polymer reagent for delivering siRNA to common cell lines
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About This Item
UNSPSC Code:
41106502
NACRES:
NA.55
grade
for molecular biology
Quality Level
usage
sufficient for 2,000 transfections (04476115001)
sufficient for 400 transfections (04476093001)
packaging
pkg of 1 mL (04476093001 [1 mg/ml])
pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])
manufacturer/tradename
Roche
concentration
1 mg/mL
technique(s)
transfection: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Short interfering RNA (siRNA) can be directly introduced into cells by transfection. A lipid-based transfection reagent, such as X-tremeGENE siRNA Transfection reagent, can provide a convenient, reliable, and efficient vehicle for delivering siRNAs into animal cells, enabling the study of cellular and functional consequences of gene knockdown.
This innovative reagent forms a complex with siRNA, as well as with mixtures of siRNA and plasmid DNA (cotransfection), and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps: mix and incubate diluted transfection reagent with diluted siRNA, then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity, it can be used without media changes. The product is animal-component free.
Contents
Solution filtered through 0.2 μm pore size membrane, supplied in polypropylene tubes.
This innovative reagent forms a complex with siRNA, as well as with mixtures of siRNA and plasmid DNA (cotransfection), and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps: mix and incubate diluted transfection reagent with diluted siRNA, then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity, it can be used without media changes. The product is animal-component free.
Contents
Solution filtered through 0.2 μm pore size membrane, supplied in polypropylene tubes.
Application
X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines, such as HT29, a human adenocarcinoma cell line.
Features and Benefits
- Knock down gene expression over 90% in many different cell types.
- Maximize experimental flexibility with a single reagent for siRNA- and cotransfection-based gene-knockdown experiments.
- Produce meaningful results using a reagent that exhibits low cytotoxicity, ensuring that the cellular effects you observe are due to the transfected siRNA rather than the transfection procedure.
- Work with or without serum, avoiding medium changes (e.g., to serum-free medium) before or after transfection.;
X-tremeGENE siRNA Transfection Reagent is a proprietary blend of lipids and other components, free of animal products.
Quality
Activity assay: X-tremeGENE siRNA Transfection Reagent (1 - 2.5 μl) is combined with siRNA (0.1 - 0.35 μg) that is specific for the HPRT housekeeping gene. The mixture is used to transfect HEK-293 cells (in a monolayer, 30 - 50% confluent) in the presence of 10% fetal bovine serum (FBS). Following transfection, the decrease of HPRT mRNA cells is determined with the LightCycler® Real-Time PCR System. A knockdown efficiency of 70 - 95% is typically observed when mRNA is measured.
Cytotoxicity analysis: HEK-293 cells are exposed to siRNA/X-tremeGENE siRNA Reagent complexes for 72 hours in the presence of serum, without a media change. Cytotoxicity is then tested by analyzing the cells with the WST-1 Cell Proliferation Reagent (Roche).
Cytotoxicity analysis: HEK-293 cells are exposed to siRNA/X-tremeGENE siRNA Reagent complexes for 72 hours in the presence of serum, without a media change. Cytotoxicity is then tested by analyzing the cells with the WST-1 Cell Proliferation Reagent (Roche).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
X-tremeGENE is a trademark of Roche
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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